next generation library preparation and sequencing (ngs) Search Results


whole genome bisulfite sequencing (wgbs)  (LC Sciences)
90
LC Sciences whole genome bisulfite sequencing (wgbs)
Identification of BM cfRNA deriving mainly from breast-derived cells. RNAs of BM cfRNAs and BM cellular RNA from three different donors (donors 7, 8, and 9) were subjected to RT-PCR to detect mRNA levels of (a) highly expressed genes in breast-related and (b) highly expressed genes in blood-related cells. The graphs show normalized −ΔCt values for the indicated genes (described in detail in Supplemental Text). All error bars denote SEM, n = 3. (c) The heat map of methylation levels in every identical reliable slide window of different samples. <t>WGBS</t> data was analyzed with default parameters (1000 bp slide windows, 500 bp overlap). For accuracy, only slide windows with more than 3000 total C reads (methylated and unmethylated) were regarded as reliable for comparison between samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order across all chromosomes of the gDNA6 sample. (d) The chromosome-separated heat map of methylation levels in identical reliable slide windows of different samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order in each chromosome of the gDNA6 sample.
Whole Genome Bisulfite Sequencing (Wgbs), supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc07354639-77-3-11?v=LC+Sciences
Average 90 stars, based on 1 article reviews
whole genome bisulfite sequencing (wgbs) - by Bioz Stars, 2026-08
90/100 stars
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custom panel of mkrn3 and dlk1 next-generation sequencing (ngs) with deletion/duplication testing  (Fulgent Genetics)
90
Fulgent Genetics custom panel of mkrn3 and dlk1 next-generation sequencing (ngs) with deletion/duplication testing
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Custom Panel Of Mkrn3 And Dlk1 Next Generation Sequencing (Ngs) With Deletion/Duplication Testing, supplied by Fulgent Genetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc07324050-92-17-26?v=Fulgent+Genetics
Average 90 stars, based on 1 article reviews
custom panel of mkrn3 and dlk1 next-generation sequencing (ngs) with deletion/duplication testing - by Bioz Stars, 2026-08
90/100 stars
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one-stop solution to next-generation sequencing (ngs) data processing, analysis, and interpretation  (AccuraScience LLC)
90
AccuraScience LLC one-stop solution to next-generation sequencing (ngs) data processing, analysis, and interpretation
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
One Stop Solution To Next Generation Sequencing (Ngs) Data Processing, Analysis, And Interpretation, supplied by AccuraScience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc04304724-36-8-4?v=AccuraScience+LLC
Average 90 stars, based on 1 article reviews
one-stop solution to next-generation sequencing (ngs) data processing, analysis, and interpretation - by Bioz Stars, 2026-08
90/100 stars
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ngs library preparation and sequencing  (Integragen sa)
90
Integragen sa ngs library preparation and sequencing
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Ngs Library Preparation And Sequencing, supplied by Integragen sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc11161510__41698_2024_616_MOESM1_ESM-37-4-8?v=Integragen+sa
Average 90 stars, based on 1 article reviews
ngs library preparation and sequencing - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
library preparation and ngs sequencing  (Snpsaurus LLC)
90
Snpsaurus LLC library preparation and ngs sequencing
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Library Preparation And Ngs Sequencing, supplied by Snpsaurus LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc08623036-51-61-53?v=Snpsaurus+LLC
Average 90 stars, based on 1 article reviews
library preparation and ngs sequencing - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
ngs library preparation and sequencing  (Singlera Inc)
90
Singlera Inc ngs library preparation and sequencing
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Ngs Library Preparation And Sequencing, supplied by Singlera Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc06373107-103-7-11?v=Singlera+Inc
Average 90 stars, based on 1 article reviews
ngs library preparation and sequencing - by Bioz Stars, 2026-08
90/100 stars
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ngs library preparation and high-throughput sequencing  (Xcelris Labs Ltd)
90
Xcelris Labs Ltd ngs library preparation and high-throughput sequencing
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Ngs Library Preparation And High Throughput Sequencing, supplied by Xcelris Labs Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc11061124-71-4-9?v=Xcelris+Labs+Ltd
Average 90 stars, based on 1 article reviews
ngs library preparation and high-throughput sequencing - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
exome enrichment, preparation of sequencing libraries and ngs  (StarSEQ GmbH)
90
StarSEQ GmbH exome enrichment, preparation of sequencing libraries and ngs
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
Exome Enrichment, Preparation Of Sequencing Libraries And Ngs, supplied by StarSEQ GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/pmc11955635-335-7-10?v=StarSEQ+GmbH
Average 90 stars, based on 1 article reviews
exome enrichment, preparation of sequencing libraries and ngs - by Bioz Stars, 2026-08
90/100 stars
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high throughput dna extraction, wga and targeted next generation sequencing (ngs)  (LGC Genomics GmbH)
90
LGC Genomics GmbH high throughput dna extraction, wga and targeted next generation sequencing (ngs)
Families with <t>MKRN3</t> deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.
High Throughput Dna Extraction, Wga And Targeted Next Generation Sequencing (Ngs), supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+library+preparation+and+sequencing+%28ngs%29/10__1007_slash_s11825___013___0376___x-3818-0-14?v=LGC+Genomics+GmbH
Average 90 stars, based on 1 article reviews
high throughput dna extraction, wga and targeted next generation sequencing (ngs) - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Identification of BM cfRNA deriving mainly from breast-derived cells. RNAs of BM cfRNAs and BM cellular RNA from three different donors (donors 7, 8, and 9) were subjected to RT-PCR to detect mRNA levels of (a) highly expressed genes in breast-related and (b) highly expressed genes in blood-related cells. The graphs show normalized −ΔCt values for the indicated genes (described in detail in Supplemental Text). All error bars denote SEM, n = 3. (c) The heat map of methylation levels in every identical reliable slide window of different samples. WGBS data was analyzed with default parameters (1000 bp slide windows, 500 bp overlap). For accuracy, only slide windows with more than 3000 total C reads (methylated and unmethylated) were regarded as reliable for comparison between samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order across all chromosomes of the gDNA6 sample. (d) The chromosome-separated heat map of methylation levels in identical reliable slide windows of different samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order in each chromosome of the gDNA6 sample.

Journal: BioMed Research International

Article Title: Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer

doi: 10.1155/2020/8126176

Figure Lengend Snippet: Identification of BM cfRNA deriving mainly from breast-derived cells. RNAs of BM cfRNAs and BM cellular RNA from three different donors (donors 7, 8, and 9) were subjected to RT-PCR to detect mRNA levels of (a) highly expressed genes in breast-related and (b) highly expressed genes in blood-related cells. The graphs show normalized −ΔCt values for the indicated genes (described in detail in Supplemental Text). All error bars denote SEM, n = 3. (c) The heat map of methylation levels in every identical reliable slide window of different samples. WGBS data was analyzed with default parameters (1000 bp slide windows, 500 bp overlap). For accuracy, only slide windows with more than 3000 total C reads (methylated and unmethylated) were regarded as reliable for comparison between samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order across all chromosomes of the gDNA6 sample. (d) The chromosome-separated heat map of methylation levels in identical reliable slide windows of different samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order in each chromosome of the gDNA6 sample.

Article Snippet: miRNA sequencing and whole genome bisulfite sequencing (WGBS) were performed by LC Sciences (Houston, TX, USA).

Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Methylation, Comparison

Families with MKRN3 deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Heterozygous Deletions in MKRN3 Cause Central Precocious Puberty Without Prader-Willi Syndrome

doi: 10.1210/clinem/dgaa331

Figure Lengend Snippet: Families with MKRN3 deletions. Squares, male; circles, female. Filled symbols denote CPP phenotype; black center dots indicate unaffected carriers based on genotype; blank symbols denote unaffected individuals with normal or unknown genotype. Abbreviations: WT, wild type; -, deletion of locus: left, MKRN3; right, 15q11.2 locus containing MKRN3, MAGEL2, and NDN.

Article Snippet: DNAs from 15 (n = 15 of 16; 93.8%) probands were sent for a custom panel of MKRN3 and DLK1 next-generation sequencing (NGS) with deletion/duplication testing (Fulgent Genetics, Temple City, CA).

Techniques:

Peripheral blood qPCR results for Family A: proband (III.1); father (II.1); paternal grandmother (I.2). Genomic DNA expression values are shown for 3 different MKRN3 primer sets (MKRN3-E1, MKRN3-E1B, and MKRN3-E1C) and control chromosomes (Autosomal [AC] and X chromosome [XC]), expressed as quantity relative to female control DNA (each graph, right).

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Heterozygous Deletions in MKRN3 Cause Central Precocious Puberty Without Prader-Willi Syndrome

doi: 10.1210/clinem/dgaa331

Figure Lengend Snippet: Peripheral blood qPCR results for Family A: proband (III.1); father (II.1); paternal grandmother (I.2). Genomic DNA expression values are shown for 3 different MKRN3 primer sets (MKRN3-E1, MKRN3-E1B, and MKRN3-E1C) and control chromosomes (Autosomal [AC] and X chromosome [XC]), expressed as quantity relative to female control DNA (each graph, right).

Article Snippet: DNAs from 15 (n = 15 of 16; 93.8%) probands were sent for a custom panel of MKRN3 and DLK1 next-generation sequencing (NGS) with deletion/duplication testing (Fulgent Genetics, Temple City, CA).

Techniques: Expressing, Control

Summary of published deletions in PWS-imprinting center region on chromosome 15. Dotted lines represent the borders of the MKRN3 locus. Shaded grey regions in bars represent the coordinates of published deletions. Left side: letter (present study, bold) or number (references cited) assigned to each case; right side: diagnoses published; bottom: scale indicating chromosome 15 position, band, with genes involved below (GRCh37/hg19). Abbreviations: CPP, central precocious puberty; PWS, Prader-Willi syndrome; Chr, chromosome.

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Heterozygous Deletions in MKRN3 Cause Central Precocious Puberty Without Prader-Willi Syndrome

doi: 10.1210/clinem/dgaa331

Figure Lengend Snippet: Summary of published deletions in PWS-imprinting center region on chromosome 15. Dotted lines represent the borders of the MKRN3 locus. Shaded grey regions in bars represent the coordinates of published deletions. Left side: letter (present study, bold) or number (references cited) assigned to each case; right side: diagnoses published; bottom: scale indicating chromosome 15 position, band, with genes involved below (GRCh37/hg19). Abbreviations: CPP, central precocious puberty; PWS, Prader-Willi syndrome; Chr, chromosome.

Article Snippet: DNAs from 15 (n = 15 of 16; 93.8%) probands were sent for a custom panel of MKRN3 and DLK1 next-generation sequencing (NGS) with deletion/duplication testing (Fulgent Genetics, Temple City, CA).

Techniques: